OPTIMIZATION OF GROWTH OF FUNGUS ON VARIOUS GROWING

Abstract

Postharvest fungi can thrive if environmental conditions support for growth and development. On a laboratory scale, the type of media is closely related to the ability of the fungus to grow optimally. An appropriate medium is a medium that supports all of the compounds required for growth and development of the fungus. Various media for isolation and identification are DCPA,DG18,CYA,CY20S. This study aims to determine the growth of Fusarium verticilloides, Aspergillus flavus, and Eurotium repens on some media. The inoculum used is pure Fusarium, Aspergillus and Eurotium. Inoculum grown in various media DCPA,DG18,CYA,CY20S, each media made as many as 3 replications. The medium is then incubated at room temperature for several days. Observation of colony diameter were observed at 2 x 24 hours and 5 x 24 hours. DG18 media is a common medium for the isolation of postharvest fungi, especially that ae xerophilic. DCPA is medium for the isolation of filed fungi, the Fusarium genus. Observation of colony growth on CYA medium showed isolate Fusarium verticilloides, and Aspergillus flavus grow well at 2 x 24 hours and 5 x 24 hours, in contrast to Eurotium repens that grow with less than maximum. The CY20S media is particularly suitable for xerophilic fungi that grows maximally on high sugar content. Based on the observations of the three isolates used grow well in this media. Based on the results of activity, Fusarium verticilloides, fungus after 5 x 24 hours of incubation was best grown on CYA medium (75,12 mm)meanwhile Aspergillus flavus (81,87 mm), and Eurotium repens (44,75 mm) on CY20S medium. Aspergillus flavus has the highest gowth value compared to the other two isolates on the most suitable medium for each isolate.